ig lambda secondary ig  (SouthernBiotech)

Journal: F1000Research

Article Title: Excess CD40L does not rescue anti-DNA B cells from clonal anergy

doi: 10.12688/f1000research.2-218.v2

Figure Lengend Snippet: Spleen cells from 11- to 34-wk-old wild type (WT), 3H9, and CD40L/3H9 mice were stained for B220 ( A and B ), Ig λ1 chain ( A and B ) and Ig λ chain ( B ), and B220 + cells were analyzed by flow cytometry. Percentages of λ1 + cells in total B220 + cells ( A ), percentages of λ + cells in total B220 + cells ( B , upper panel) and percentages of λ1 + cells in total λ + cells ( B , lower panel) are indicated (left panels). Representative data from three independent experiments. Graphs show mean ± SD, three mice per genotype (right panels). * p<0.05, ** p<0.01, ns = not significant.

Article Snippet: Alexa Fluor 647-conjugated rat anti-mouse B220 (RA3-6B2, BioLegend, USA) (1:100), Pacific Blue-conjugated anti-mouse Ig λ1 (LS-136, a kind gift of Dr. G. Kelsoe at Duke University) (1:100) and FITC-conjugated goat anti-mouse Ig λ chain antibody (Cat No. 1060-02, Southern Biotech, USA) (1:1000).

Techniques: Staining, Flow Cytometry